Tuesday, April 26, 2011

Coffee, Tea, or Me?

Another late night affair with my Tuesday-Thursday articles, with coffee and tea as my bright-eyed cohorts. Today's strapping gent is "Listeria monocytogenes ActA-mediated escape from autophagic recognition" by Yoshikawa, et al.
If only the figures were not sprawled out over a billion pages and referenced 5 pages back.
If only the researchers did less studies and the paper were shorter. 22 pages shorter.
If only it didn't eat ink cartridges. But I printed this one on low settings. I can't see crap in the figures. Mistake?
I painted my nails today. Bali Mist. I see nothing Bali or Mist about the color. All I know is that I dented my thumb paint on the edge of my calculator while prying out dying AAA batteries, and I have no burning desire to fix that.
If only my attention span carried past the duration of half a sentence.
If only the paper consisted of more words and less 3-letter+number(s) names.

"After 2 h of infection, p62 colocalized with ubiquitin and GFP–LC3 around ΔactA2 bacteria (Fig. 3a, b). p62–/– MEFs stably expressing GFP–LC3 (p62–/–) were infected with ΔactA2 bacteria for 2 h. The amount of ubiquitin associated with ΔactA2 bacteria in p62–/– MEFs was similar to that in p62–/– MEFs stably expressing GFP–LC3 and p62-3×Myc (p62–/–/p62); however, the amount of LC3 associated with ΔactA2 bacteria in p62–/– MEFs was less than half that in p62–/–/p62 MEFs (Fig. 3c; Supplementary Information, Fig. S4a)." -Yoshikawa et al.
"Expression of GFP–Q79C or GFP–ActAC–Q79C, in COS‑7 cells, induced the formation of ubiquitin- and p62-positive aggregates (Fig. 4d, e). However, on expression of GFP–ActAFL–Q79C or GFP–ActAN–Q79C, in COS‑7 cells, neither ubiquitin nor p62 was found to colocalize with the GFP signal (Fig. 4b–e). Importantly, in COS‑7 cells depleted of Arp2 and VASP production by their respective siRNAs, GFP–ActAFL–Q79C and GFP–ActAN–Q79C formed aggregates as did GFP–Q79C and GFP– ActAC–Q79C (Supplementary Information, Fig. S5a, b), indicating that binding of the Arp2/3 complex and VASP to their respective domains in ActA prevents aggregate formation. We used another aggregate-prone construct, GFP–170*, a nonpoly‑Q protein that contains GFP fused to an internal segment of the Golgi membrane protein 170, which has also been used previously as a model substrate for aggregate formation." -Yoshikawa et al.

...Hello, coffee. Short attention span ftl. =[ This stuff makes no sense if you nod off every other word.

I suck at reading. But this is our last article for the semester! *rejoices!* I actually found a lot of the previous articles to be extremely fascinating. I'm not feeling this one! Time to plow through the remaining pages!


I haven't been too productive lately. I'll blame the gloomy weather on this one. Oh, unsuspecting scapegoat! And if it were brighter and sunnier, I would probably blame that as well. c; But I have a new dress that I really want to wear. Hurry, sun! Except it's gonna be rainy and cloudy all week....and all next week. NOOOOOOO

In other news, I am slowly falling under the yucky spell of caffeine wizardry. I have no desire to suffer from caffeine withdrawal, nor do I have a desire to throw copious amounts of money at Momodomo. But I guess $1 a cup isn't as offensive as whatever Starbucks charges! I shall be investing in a coffee machine next year for instant access to cheap and crappy coffee. That's fine because coffee tastes like crap anyways!

Edit: Oh balls, this article makes perfect sense now. VICTOLY!!! Took 2 reads.

    Sunday, April 24, 2011

    Phantogram: Eyelid Movies

    Officially formed in 2007 by Josh Carter and Sarah Barthel, Phantogram looks like your typical indie band. Barthel's hair screams mod, and the band's genre classification seems arrogant. Fortunately, this NY duo proves to be more than your typical newcomer with their debut album. Eyelid Movies (2010) combines various elements of electronica, street beats, indie pop, dream pop, shoegaze, hip-hop, and trip-hop to create a delectable musical experience swimming with synthy elements, brassy interjections, effective sampling, and solid beats, topped with dreamy vocals.

    While the entire album is covered in a slight shade of grey, Phantogram does a lovely job incorporating both darker and lighter tracks, resulting in a strongly atmospheric, yet never monotonous album that takes influences from staple artists in various genres and then melds these elements into something fresh and interesting. Carter's rougher, and at times less impressive, vocals appear in stark contrast with Barthel's soft and dreamy singing, making for an interesting push-and-pull throughout the album that follows the the same push-and-pull feel of the tracks, which ultimately resolves itself in an exhilarating finish. Lyrically, you'll find no sweet love songs or strong social commentary, but instead, the raw unfoldings of rejection, dependency, melancholy, and the likes.

    Unmistakably indie, but not pretentiously so, Eyelid Movies is a great debut album that you may find yourself listening to multiple times before it suddenly pounces out of mediocrity and latches onto your eardrums faster than its tracks can alternate between the raw and edgy to the flowing and dreamy.

    -Rating: 9/10



    Album: Eyelid Movies
    Year: 2010
    Artist: Phantogram

    01. Mouthful of Diamonds
    02. When I'm Small
    03. Turn It Off
    04. Running from the Cops
    05. All Dried Up
    06. As Far as I Can See
    07. You Are the Ocean & I'm Good at Drowning
    08. Bloody Palms
    09. Futuristic Casket
    10. Let Me Go
    11. 10,000 Claps


    At first glance, Eyelid Movies contains a few catchy songs right that might mildly interest a fan of the genre on their first listen. The real beauty of this album really takes a few listens--it definitely did for me, as I only enjoyed Let Me Go and When I'm Small at first and really didn't appreciate the complexity of the pieces. I think it's been a month or so, and the album has finally settled in. c; On a side note, I don't really care for their videos. I might even say that they make the songs a lot less enjoyable, but eh!

    I decided to leave out scores for my least favorite tracks.


    01. Mouthful of Diamonds - 7/10 - Released in 2009 as a single, Mouthful of Diamonds appears to be one of the more well-known tracks by this duo. While this track isn't my favorite, I must admit that the dreampop and headbobability feel of this song, paired with Barthel's dreamy vocals, makes for a good listen.
    -Listen-

    02. When I'm Small - 9.5/10 - This track is easily one of my favorites from the album and also happens to be the first track that drew me to Phantogram. When I'm Small is a forward-moving, beat-driven track that builds up for over three minutes, alternating between strong, beat-driven backing and flowing interludes, leading to a beautiful 40-second finish, which I would have to say is one of the finest moments in the entire album. The ultimate treat is hearing Barthel sing, "I'd rather die; I'd rather die than to be with you" at the climax, followed by a downpour of sheer epicness that can only be described by the song title, "Jigsaw Falling into Place." 
    -Listen-

    06. As Far As I Can See - 8.5/10 - This track is one of the better tracks on the album that demonstrates Phantogram's ability to combine a variety of influences to create a catchy, effective track that cannot be adequately described under any label.  Their heavy use of brass sampling, combined with various hip-hop and trip-hop elements under, of course, dreamy vocals, makes for a lush listen that is smooth and delicate, yet solid, soulful, and edgy.
    -Listen-

    07. You Are the Ocean & I'm Good at Drowning -6.5/10

    08. Bloody Palms - 7.5/10 

    09. Futuristic Casket - 8.5/10 - A powerhouse of a track, Futuristic Casket starts off strong, with an unmistakably raw, industrial and urban attitude. And then it slips into shoegaze and before you can even catch the seamlessly gradual transition. Carter's vocal effects fit very well with the song 's two distinct styles.

    10. Let Me Go - 9/10 - A restless turmoil of sharp percussion, brass with an attitude, and flowing piano makes for something that feels like a an urban waltz with sweet honey bite. Throw on Barthel's clear, confident vocals, and you have a beautiful juxtaposition. I'm not even going to begin to attempt to list the various styles incorporated into this piece.
    -Listen-

    11. 10,000 Claps - 8.5/10 - Brooding, pensive, and subdued, 10,000 Claps is a lovely, lovely close to the album. The album funnels down from the lush, beat-filled, and oftentimes edgy landscape painted by all the previous tracks, and finishes on this one anomaly of a track. Dreamy, lonely, melancholic, and sparse, the simple piano backing and Barthel's vocals encourage retrospection, introspection, and reflection. It is definitely a beautiful curtain-closer, except the curtain is made of the softest, lushest, red velvet imaginable, and it falls--achingly, slowly, freely--before a stage of silent, grey, flickering dancers. And as the curtain reaches the end of its descent, it takes a final breath, and there is applause. 
    -Listen-




    Album: 9/10


    The Ups: Interesting, not monotonous (which can be an issue for others in this genre), effective hybrid of many styles, catchy, complex
    The Downs: Some tracks don't feel as polished as they could be, some ideas can be more further developed, takes a long time to appreciate


    He's telling you to listen.

    Monday, April 11, 2011

    5:33am

    have an exam at 10 and 12.
    CRAPCRAPCRAPCRAP
    This is keeping me awake:





    She's quite lovely.
    Oh, and I acquired a large MomoDomo coffee as well as a sweet tea.
    I'm not even jittering.

    Also, I've recently discovered that I prefer Pandora over last.fm :D