Coffee, Tea, or Me?

Another late night affair with my Tuesday-Thursday articles, with coffee and tea as my bright-eyed cohorts. Today's strapping gent is "Listeria monocytogenes ActA-mediated escape from autophagic recognition" by Yoshikawa, et al.
If only the figures were not sprawled out over a billion pages and referenced 5 pages back.
If only the researchers did less studies and the paper were shorter. 22 pages shorter.
If only it didn't eat ink cartridges. But I printed this one on low settings. I can't see crap in the figures. Mistake?
I painted my nails today. Bali Mist. I see nothing Bali or Mist about the color. All I know is that I dented my thumb paint on the edge of my calculator while prying out dying AAA batteries, and I have no burning desire to fix that.
If only my attention span carried past the duration of half a sentence.
If only the paper consisted of more words and less 3-letter+number(s) names.

"After 2 h of infection, p62 colocalized with ubiquitin and GFP–LC3 around ΔactA2 bacteria (Fig. 3a, b). p62–/– MEFs stably expressing GFP–LC3 (p62–/–) were infected with ΔactA2 bacteria for 2 h. The amount of ubiquitin associated with ΔactA2 bacteria in p62–/– MEFs was similar to that in p62–/– MEFs stably expressing GFP–LC3 and p62-3×Myc (p62–/–/p62); however, the amount of LC3 associated with ΔactA2 bacteria in p62–/– MEFs was less than half that in p62–/–/p62 MEFs (Fig. 3c; Supplementary Information, Fig. S4a)." -Yoshikawa et al.

"Expression of GFP–Q79C or GFP–ActAC–Q79C, in COS‑7 cells, induced the formation of ubiquitin- and p62-positive aggregates (Fig. 4d, e). However, on expression of GFP–ActAFL–Q79C or GFP–ActAN–Q79C, in COS‑7 cells, neither ubiquitin nor p62 was found to colocalize with the GFP signal (Fig. 4b–e). Importantly, in COS‑7 cells depleted of Arp2 and VASP production by their respective siRNAs, GFP–ActAFL–Q79C and GFP–ActAN–Q79C formed aggregates as did GFP–Q79C and GFP– ActAC–Q79C (Supplementary Information, Fig. S5a, b), indicating that binding of the Arp2/3 complex and VASP to their respective domains in ActA prevents aggregate formation. We used another aggregate-prone construct, GFP–170*, a nonpoly‑Q protein that contains GFP fused to an internal segment of the Golgi membrane protein 170, which has also been used previously as a model substrate for aggregate formation." -Yoshikawa et al.

...Hello, coffee. Short attention span ftl. =[ This stuff makes no sense if you nod off every other word.

I suck at reading. But this is our last article for the semester! *rejoices!* I actually found a lot of the previous articles to be extremely fascinating. I'm not feeling this one! Time to plow through the remaining pages!

I haven't been too productive lately. I'll blame the gloomy weather on this one. Oh, unsuspecting scapegoat! And if it were brighter and sunnier, I would probably blame that as well. c; But I have a new dress that I really want to wear. Hurry, sun! Except it's gonna be rainy and cloudy all week....and all next week. NOOOOOOO

In other news, I am slowly falling under the yucky spell of caffeine wizardry. I have no desire to suffer from caffeine withdrawal, nor do I have a desire to throw copious amounts of money at Momodomo. But I guess $1 a cup isn't as offensive as whatever Starbucks charges! I shall be investing in a coffee machine next year for instant access to cheap and crappy coffee. That's fine because coffee tastes like crap anyways!

Edit: Oh balls, this article makes perfect sense now. VICTOLY!!! Took 2 reads.

5:33am

I have an exam at 10 and 12.
CRAPCRAPCRAPCRAP

This is keeping me awake:

She's quite lovely.
Oh, and I acquired a large MomoDomo coffee as well as a sweet tea.
I'm not even jittering.

Also, I've recently discovered that I prefer Pandora over last.fm :D 

Organizing My Folders Means Discovery

For the upcoming semester of school, I've finally decided to organize my folders and stick my College folder into the Documents folder. Before doing so, however, I noticed that my documents folder was disgustingly cluttered and that I had picture and music folders within my documents folder, despite the presence of C:\Users\Jenny\Music and C:\Users\Jenny\Pictures (and C:\Users\Jenny\Piccys.....don't ask).

Ahh, jmol. Why do I still have you? Ahh, hidden folders, why do I still have you set as visible?

So while I was cleaning out my documents folder, which included both reorganizing and deleting old things that I no longer needed or knew existed, I stumbled upon some writing I did back in the day. It pains me to say that my vocabulary and writing skills have declined precipitously since my early high school years, not that I had much MAD SKILLZ to begin with.  

I'd say the most amusing part was trying to remember what the fack I was thinking back then. I don't even remember writing half of the stuff I did.

And here are the contents of a random text file titled smoke.txt (wtfack a poem? But I hate poems.):

who are you, the silent brooder,
to whom the shark-fin waves speak?
For your heart is that of a churning maelstrom
And your smile slow and twisted,
like a too-soft banana skin
slowly being peeled back,
curling over like tendrils of smoke
over a sluggish metropolis

The sharp scent of your cheap cologne
cuts through the stagnant air on a summer evening,
much like ink bleeding through a diner napkin
I sense impatience--or perhaps it is just play of thought--
playing through your cloudy mind,
tapping out its irregular, staccato rhythmn
from a slick, lacquer red heel,
stiff straps lined with worry-wrinkles

And your vacant stare is that of the blind man
as you look to the tall, cigarette smokestacks
before a hazy sunset of some rich exotic incense
And the sun is a dull lantern
behind silk screens and screen doors

A single moth the color of white paint peeling from
cracked wooden walls
toys with danger
as it dances in the candlelight

.....WHAT?

Here's another trippy one:

It rises from the ashes
of a metropolitan wasteland.
It floods minds with flourescent light,
opens UV-protected eyes,
clears the fog off weather-insulated souls.

Upstairs, a great mind turns,
mechanical heart ticks away into the future
as neon lights and falling confetti
float in serenity
beneath the luminescence of time.

The man realizes that the great Moth
will die in burning passion
as it pursues destiny,
as it seeks the light.

And so
the man,
confetti in
his hair, his ears, his eyes,
stumbles into the chamber of darkness.

Did I hate buildings or something?
I also found a story involving an axe and yellow plates. But that one's a bit long, and I kind of want to go through it first. O.o TRIPPY SCHIZZ, and I didn't do drogas!

Anyways, organizing adventure: mild success.
Rule of thumb: If you don't know it's there, delete it. Of course, this applies to stuff you saved as opposed to important stuff.

Bombing the Toilet

Taco Bell.